Biochemistry, physics, and life sciences rely abundantly on various types of spectrophotometers by the top Spectrophotometer manufacturer, suppliers, and exporters. Spectrophotometers are indispensable for the immense insight it brings to various science branches.
Spectrophotometers are advanced analytical scientific instruments. It measures the intensity of light absorbed by molecules at different wavelengths, providing critical information about their composition and concentration.
Working principle of spectrophotometers: Each molecule absorbs light differently, allowing scientists to precisely quantify the concentration of substances like DNA, proteins, or chemicals. The working principle of spectrophotometers is based on measuring a sample’s absorption or transmission of light.
Parts of spectrophotometers: There are various spectrophotometers, but a spectrophotometer’s basic components include a light source, a sample holder, a monochromator or a filter, a detector, and a display or data output device.
Atico Lab Export, the top Spectrophotometer manufacturer, supplier, and exporter, provides various types of spectrophotometers. Each type of Spectrophotometer has a specific purpose and application. Let us understand what the various types of spectrophotometers are.
Types of Spectrophotometers
Spectrophotometers are analytical instruments used in chemistry and life sciences, metallurgy laboratories, and many more to measure light intensity in a sample as a function of the wavelength.
The most common types of spectrophotometers used in laboratories are:
UV-Vis Spectrophotometers
Purpose: The purpose of UV-Vis spectrophotometers is to analyze and quantify the concentration of substances in a sample by measuring the amount of light absorbed by the sample at different wavelengths. It measures light absorption in the ultraviolet (UV) and visible (Vis) regions of the electromagnetic spectrum.
Top Spectrophotometer manufacturers, suppliers, and exporters provide various types of UV-Vis spectrophotometers, which differ in design, functionality, and intended use. It includes:
- Single-beam spectrophotometers: use a single beam of light to measure the absorption spectrum of a sample. They are commonly used in educational settings and for basic laboratory applications.
- Double-beam spectrophotometers: Double-beam spectrophotometers use two beams of light, one passing through the sample and the other through a reference solution, which is used to compensate for any background interference. This provides more accurate and reliable results than single-beam spectrophotometers and is commonly used in research and industrial settings.
Besides this, we manufacture more types of spectrophotometers in this category:
- Split-beam spectrophotometers: Split-beam spectrophotometers use a beam splitter to divide the light beam into two paths. It allows for simultaneous sample and reference solutions measurement, improving the results’ accuracy and precision.
- Microplate readers: they simultaneously measure the absorption spectra of multiple samples.
- Portable spectrophotometers: they are compact and lightweight instruments for field measurements. They are commonly used in environmental and agricultural applications, such as monitoring water quality and soil nutrient levels.
Application: Some common applications of UV-Vis spectrophotometers include:
- Quantification of DNA and protein
- Analysis of ingredients in pharma samples
- Determine the level of pollutants in water samples for environmental analysis.
- Analyze the concentration of various compounds in food and beverages, such as caffeine in coffee.
- Analysis of the concentration of petroleum products
Infrared (IR) Spectrophotometers
IR spectrophotometers analyze samples in the infrared region of the electromagnetic spectrum. They are widely used in analytical chemistry to analyze organic compounds like hydrocarbons and polymers. These spectrophotometers utilize infrared light that interacts with a sample’s molecules by causing them to vibrate, generating a unique signal that reflects its chemical properties.
Application: They have a vast application in the following fields:
- Identify unknown compounds and identify and analyze the chemical composition of various chemical materials, such as polymers, plastics, and pharmaceuticals.
- They are used in forensic investigations to analyze and identify unknown substances.
- They are commonly used to analyze the composition of petroleum products, such as crude oil, gasoline, and diesel fuel.
Atomic Absorption Spectrophotometer
Purpose: the state of the art technology by a top Spectrophotometer manufacturer and supplier brings the most advanced Atomic Absorption Spectrophotometer to the market. These advanced instruments offer exceptional sensitivity and accuracy for trace metal analysis and analyzing the concentration of metal ions in a sample.
Applications: To know more about AA spectrophotometers, it is best to learn more about their uses:
- Ideal for environmental analysis to analyze soil, water, and air samples for heavy metal pollutants.
- Determine the concentration of metal ions in pharmaceutical formulations.
- Measure the concentration of metal ions in food and beverage samples, such as lead in wine.
- It helps to measure the concentration of trace metals in blood and urine samples.
- One of the primary uses is the analysis of rocks and minerals for the presence of metal ions.
Fluorescence Spectrophotometer
Purpose: It measures the intensity and wavelength distribution of fluorescent light emission. They analyze the fluorescence properties of a sample. With this analysis, we ascertain the composition, structure, and properties of complex molecules such as proteins or nucleic acids.
Applications: Medical research laboratories and various industries use them for the following:
- It is a great tool for studying the structure and function of biological molecules such as proteins, DNA, and RNA.
- An important tool in the drug discovery process, as it allows researchers to identify potential drug candidates.
- Material science labs use Fluorescence spectrophotometers to study the electronic and optical properties of materials such as polymers, semiconductors, and nanoparticles.
Circular Dichroism (CD) Spectrophotometers
Purpose: Top Spectrophotometer manufacturers manufacture them to give scientists an unprecedented view into the three-dimensional structures of proteins, nucleic acids, and other biological macromolecules. These spectrophotometers can detect the minutest changes in chiral molecules’ orientation or configuration.
Applications: The most exquisite applications of Circular Dichroism (CD) Spectrophotometers include:
- CD spectrophotometers are used to study the secondary structure of proteins, such as alpha-helices and beta-sheets.
- CD spectrophotometers are used in medical research labs to study the structure of nucleic acids, such as DNA and RNA.
- It is a vital tool for studying the chiral properties of materials, such as polymers and liquid crystals.
Connect us for the most eminent Spectrophotometer manufacturer, supplier, and exporter of A-grade quality spectrophotometers. We strive for excellence and ensure to bring nothing but the best to the market.
